بررسي پلي مورفيسم 17 نشانگر ميكروساتلايت در جمعيت گوسفند نژاد بلوچي

Polymorphism Analysis of 15 Microsatelite Markers in Baluchi Sheep Breed

Baluchi sheep is the most dominating sheep breed in Iran, its breeding station being located in Abbas Abad, Mashad. In this study, genetic diversity was attended to through a study of polymorphism in 17 microsatellite loci in its population. Each of the loci was investigated using 145 DNA samples. Polymeroze Chan Reaction (PCR) conditions for all loci were optimized and PCR products run on polyacrylamide gels, bands being revealed by a silver staining procedure. Based on the results, all loci, except BMS4000 and OarAE54, were amplified and showed polymorphism. MCM120 (0.9232) and TGLA387 (0.6839) carried the highest and lowest Polymorphic Information Content (PIC) values respectively. The highest number of actual alleles were observed in MCM120 (21 alleles) and the least number of actual allele in MCM494 and MCM541L (9 alleles). Also it was shown that the highest and the lowest number of effective alleles belonged to CSRD144 (14.9664) and TGLA387 (3.6255), respectively. Average heterozygosity over all loci in this population was found as 0.8764. All loci, based on X2 and G2 tests, were out of Hardy-Winberg Equilibrium (HWE) while only MCM120 by G2 test stood in HWE. High genetic diversity in the population of Baluchi sheep, based on the studied loci, can be concluded from the results obtained from the present investigation.