بررسي اثر ضد ويروسي عصاره سير بر روي هرپس سيمپلكس ويروس با استفاده از روش كشت سلولي

An investigation on antiviral effect of Garlic extract on Herpes simplex virus via cell culture

Background & Aim: Herpes simplex virus is responsible for many infections in various parts of the body. Type I is the cause of two oral diseases: acute gingivostomatitis and cold sore. Many drugs have been recommended in this field; each of which has its specific side effects. Introducing a kind of herbal drug which has therapeutic properties could be a good one for this purpose. This study was designed to investigate the antiviral effect of Garlic and the use of it as an herbal drug in the treatment of HSVI infections. Methods & Materials: Hela cell culture was used for several assays in this experimental study. The required virus was collected from infected patients. After increasing the amount of virus, it was titrated by TCID50 assay via cell culture. Finally, the cytotoxic doses of Garlic extracts were determined in cell culture and the antiviral doses were assessed. Results: The doses that caused 50% of cells death in cell culture (CC50) respectively for aqueous and alcoholic extract were: 2.91mg/ml and 3.05mg/ml. The doses that caused 50% reduction in the virus cytopathic effect (IC50) were: 1.13 and 0.98mg/ml. Conclusion: It is obvious that both types of Garlic extracts had a significant antiviral effect on HSVI. On the other hand Garlic had cytotoxic effect that appeared in the higher doses than antiviral doses