مطالعه ژن كانديدا در ديابت نوع 1 : ژن IFN- لاندا

CANDIDATE GENE ANALYSIS IN T1DM: IFN-y GENE

Background: IFN-y is one of the most essential and fundamental player in initiation and development of T1DM. This mediator belongs to T Helper-l(Thl) class of cytokines and exerts stimulation and differentiation of naive T cells towards Thl cells and meanwhile inhibits differentiation and proliferation of Th2 cells. These effects are highly important in induction and progression of cell- mediated immune responses that is aberrantly operative in development of T1DM. Due to such outstanding role, numerous studies including genetic manipulation have focused on the role of this pro-inflammatory cytokine in T1DM. Methods: In a genetic association study the influence of IFN-y gene variation in position +874*T/A on development of T1DM was analysed in 248 British Caucasian T1DM patients in comparison with 119 healthy matched controls. ARMS-PCR procedure was designed for detection of the variants at allele/genotype level. Results: No significant association between IFN-y gene polymorphism and T1DM was apparent (P> 0.05). The distribution of these polymorphic variants was in Hardy-Weinberg equilibrium. Conclusion: While some studies have shown an association between the examined polymorphism of IFN-y and T1DM, our data do not support that. According to present study the selected polymorphic marker (+874*T/A) is not among the polymorphisms that governs in part genetic susceptibility to T1DM. That ^reproducibility or controversial results is a common observation in genetic studies of complex traits such as T1DM, reflecting a fragile correlation between genotype and phenotype. This study also underlines the importance of replication of association studies to confirm the previous results/interpretations.