چكيده لاتين :
Self-incompatibility and cross incompatibility between various pairs of sweet cherry cultivars is attributed to the multi-allelic locus S, expressed gametophytically. In this study the S-genotypes of 24 Iranian and foreign sweet cherries and one Duke cherry cultivar were studied using Polymerase Chain Reaction (PCR) method. Thirteen specific primers designed for amplification of Sj, S2, S3, S4, S5, S6, S7, S9, S]0, S!2, S]3, S]4 and S]6 alleles were employed. Using specific primers 35 out of 50 S-alleles were identified in studied cultivars. Two S-alleles for 13 cultivars and one S-alleles for 9 cultivars were amplified. However, no S-allele could be identified in 3 cultivars. Only S3, S4 and Si4 alleles out of 13 mentioned S-alleles were detected in Iranian sweet cherry cultivars. S3S4 was the most frequent cross incompatibility group (CIG) that observed in 40% of Iranian sweet cherry cultivars. Moreover, one new CIG was proposed as group XXV comprising ʹSorati-e-Hamadanʹ and ʹSiahe-e-Gazvinʹ cultivars with S4S]4 S-genotype. Although allele-specific PCR amplification was not capable enough to identify all S-alleles, but it provides a rapid and efficient approach for determining the S-genotypes in sweet cherry cultivars.
