مطالعه ساختار ژنتيكي جمعيت هاي Fusarium solani جدا شده از سيب زميني، كدوييان، لوبيا و نخود براساس نشانگر FAFLP

STUDY ON GENETIC STRUCTURE OF Fusarium solani POPULATIONS ISOLATED FROM POTATO, CUCURBIT, BEAN AND CHICKPEA BASED ON FAFLP MARKERS

Genetic structure of Fusarium solani isolates from four host populations was investigated. One hundred and forty-nine isolates were analyzed for their fluorescent amplified fragment length polymorphism (FAFLP) patterns. The isolates were obtained from four host populations including 60 isolates from potato, 30 isolates of F. solani f.sp. cucurbitae, 29 isolates of F. s. f.sp. phaseoli and 30 isolates of F. s. f.sp. pisi. After DNA extraction, digestion by EcoRl and Msel restriction enzymes, and ligation of adapters to DNA fragment, a preamplification PCR was done. Selective PCR amplification was performed using three primer combinations labeled with fluorescent dyes. The products were separated by capillary electrophoresis on an ABI PRISM 310 Genetic Analyzer. Analysis was performed based on 151 polymorphic markers. Polymorphic bands were analyzed by Popgen 32, GeneAlex 6.1 and NTsys-pc 2.0 software programs using UPGMA method and Nei coefficient. In general, 92% of observed molecular variance was related to within host populations and 8% variation was found among them. Genetic similarity between four mentioned host populations was more than 92%. In average genetic variation for host populations of potato, cucurbit, bean and chickpea were 0.3431, 0.3792, 0.3619 and 0.3606, respectively. In average genetic variation for all isolates was 0.3883 and this is not related to their host and geographical origin. The results indicated that the fungal isolates related to potato, cucurbits, bean and chickpea in this study, are one clonal lineage. High degree of similarity between populations, may be due to gene flow among them.