شيوع ژن هاي TEM1 و SHV1 در كلبسيلا پنومونيه توليدكننده بتالاكتامازهاي وسيع-الطيف

Prevalence of TEM1 & SHV1 genes in Kelebsiella pneumoniea with ESBL

Aims. Among Klebsiella spp. Klebsiella pneumoniae has special importance in nosocomial infections. Prevalence of strains producing extended spectrum B-lactamases (ESBL) and their resistance to (3-lactam antibiotics is increasing in recent years. Because of the importance of ESBLs in Klebsiella pneumoniae, this study focused on detection of ESBLs producing Klebsiella pneumoniae and its resistance pattern to (3-lactam antibiotics in Shahid Beheshti hospital of Kashan. Methods. This descriptive study was performed on clinical specimens isolated from Shahid Beheshti hospital of Kashan. ESBL production was investigated on isolates by double disk diffusion synergy technique. Detection of TEM1 and SHV1 genes that produce extended spectrum (3-lactamase were carried out with their specific primers by PCR. Results. From 100 Klebsiella pneuomoniae species that were isolated in this study, 32 specimens were ESBL (32%). 7 isolates out of 32 (21.8%) included TEM1 and SHV1 genes. 16 specimens (50%) had SHV1 gene and 12 specimens (37.5%) had TEM1 gene. Conclusion. The prevalence of ESBL in Klebsiella pneumoniae and its resistance pattern is noticeable, so using the combination of (3-lactam antibiotics with (3-lactamase inhibitors is suggested only in patients with serious infection. Dosage of antibiotic and admission period in hospital correlate with ESBL.