مقايسه فراواني وجود آنزيم (beta)-لاكتاماز در باكتري هاي جداسازي شده از عفونت هاي بيمارستاني

Comparing the Frequency of p-Iactamase Enzyme Existence in Isolated Nosocomial Infection Bacteria

Background and Objectives: ~-Iactame antibiotics are very important in the cure of diseases. ~-Iactamase as a virulence agent causes resistance to these antibiotics. Existence of ~-Iactamase in the pathogen bacteria can cause delay in the treatment. The aim of this research was to compare the frequency of ~-Iactamaseenzyme existence in different types of isolated nosocomial infection bacteria in Alzahra hospital, Isfahan, Iran. Materials and Methods: This laboratory study was performed in Alzahra hospital of Isfahan in 2005-2006. According to sample size estimation, 100 infection samples (blood, urine, skin) were randomly selected. Identification of bacteria was performed using microbiological methods; such as: staining, chemical test, and use of differential and selective media. To determine (3-lactamase production in bacteria, acidimetric method was used. Data was analyzed using descriptive statistics. Results: From the 100 isolated pathogen bacterias, 68.45% produced ~-Iactamase; 83.330/0 produced Staphylococcus spp. 70.950/0 produced Enterobacteriaceae spp. and 18.80/0 produced Pseudomonas 18.8%. Conclusion: Based on the results, the frequncy of (3-lactamase Enzyme existence in isolated bacteria of nosocomial infection was very high, which can be due to the increase of resistance of (3-lactam antibiotics in pathogen Bacteria.