بررسي و مقايسه اثرات ضد‌باكتريايي عصاره الكلي برگ، گل و ريشه آويشن شيرازي بر روي استافيلوكوكوس اوريوس مقاوم به متي‌سيلين

Evaluation and Comparison of Antibacterial Effects of Alcoholic Extracts of Zataria multiflora Boiss Leaves, Flowers and Root on Methicillin-Resistant Staphylococcus aureus

Background: Due to increasing drug resistance among pathogenic bacteria toward current and new generation antibiotics, researchers have been investigating to find herbal antimicrobial agents as alternative medicines. Objectives: In this study, the inhibitory effects of alcoholic extract of Zataria multiflora Boiss leaves, flowers and root on methicillin-resistant Staphylococcus aureus was evaluated and compared. Methods: 50 grams of dried thyme leaves, flowers and root were added to 250 ml hydroethanol separately, and were then distilled. The antibacterial properties of the extracts were assessed by Broth Dilution Method and after determining dried weights of the extracts per ml, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of them were obtained. Finally, by using Thin Layer Chromatography (TLC), effective compounds of the extracts were detected. Results: Thyme leaves alcoholic extract at 3/031 mg/ml and 6/062 mg/ml concentration, flowers alcoholic extract at 5/687 mg/ml and 11/375 mg/ml concentration and root alcoholic extract at 5/437 mg/ml and 10/875 mg/ml concentration, in order, were able to inhibit and kill all the strains and no difference were observed between them. Analysis of the chromatograms showed that thymol and carvacrol are the main compounds of this herb. Conclusion: However clinical application of herbal extracts and oils sounds valuable because of their less side effects in comparison with current drugs, in order to clinically use thyme alcoholic extracts, further investigations must be done in clarifying the mechanism of action of its effective compounds on microbial agents