بررسي مقاومت دارويي سويه هاي E.COLI جدا شده از عفونت هاي ادراري در شهر ايلام به روش ديسك ديفيوژن پلاسميدي آن ها

A Study of Antibiotic Resistance Pattern and Plasmid Profile of Uropathogenic Escherichia coli Isolated in Ilam(western Iran)

Introduction: Assessment of antimicrobial resistance patterns and plasmid profile of 100 uropathogeic E. coli isolated from outpatient with UTI in Ilam(western Iran) was designed as the goal of our research . Materials Sc Methods: Urinary samples isolated from symptomatic UTI cases were identified by routine methods. Antimicrobial susceptibility testing was performed by Kirby Bauer disc diffusion method according to CLSI guidelines. Plasmid extraction kit was used for plasmid extraction. Findings: All the isolated samples proved sensitive to meropenem. The resistance rates of E.coli were as follows: 81% for amoxycillin, 64% for tetracycline^ 1% for ciprofloxacin, 16% for cefepime and ceftazidime, 15% for ceftriaxone, cotrimoxazole and tubramycin, 12% for gentamicin, 5% for nitrofurantoin, and 1% for amikacin. Plasmid profile investigations showed the isolated E.coli carried plasmid ranging from 21KD to 1/5KD in size. The number of plasmid in each of these isolates ranged from one to eight of different sizes. The E.coli isolates contained a common plasmid with a molecular weight of 21 KD which proved to enjoy resistance to, amoxicillin and possibilly tetracycline. Discussion <£ Conclusion: There are doubts as to the role of tetracycline and amoxicillin for empiric therapy of UTI. Meropenem amikacin, nitrofurantoin, gentamicyn and ciprofloxacin are active against the great majority of UTI associated E.coli. 1KD R-plasmid reservoir may contribute to the spread of amoxicillin and probablly tetracycline resistance in our region