آناليز پلي مورفيسم DNA القاء شده در موشها بوسيله تشعشعات راديواكتيو حاد و مزمن

Analysis of induced DNA polymorphism in mice exposed to acute and chronic radioactive irradiations

Results of many studies have shown that in animal and plant populations, naturally exposed to radiation there are genetic instability. In this study, our goal was evaluation of RAPD polymorphism in offspring (Fi generation) from parents exposed to acute (3Gy) and chronic (total dose was 37cGy) irradiation. RAPD method widely use for classification of breeds, lines, sorts and cultures and also drawing up phylogenetic maps. In the view of radiogenetic monitoring the positive property of RAPD method is possibility of rapid scanning of genome descendants irradiated parents and no prior genomic sequence information. Male CBA/lac and BALB/c mice ar,^ female hybrid (CBA/lacxc57/black) mice are used in study. The results of this experiment indicated that using this primers give the most variability of RAPD markers in offspring from male parents exposed to gamma-radiation (3Gy) 3 months before mating. We also observed increased level of DNA polymorphism in descendants of parents exposed to chronic gamma-radiation. Our results demonstrated that level of polymorphism in irradiated mice is 27-50% more than non-irradiated mice. Usim ,! u\> primer series (759, 760, Ml (761 and 763), 765 and M2 (766, 768, 769 and 775) cl-Jc possible to receive significant increase in mean frequency of new bands in offspringʹs treatment group that allow us to use this system in future investigations for studying induction of genomic instability.