همانه سازي و انتقال ژن فعال كننده پلاسمينوژن بافتي (tPA) با منشاء انساني به گياه توتون

Cloning and Transformation of human tissue Plasminogen Activator (t-PA) gene in Tobacco Plants

Plants offer a promising alternative to microbial fermentation and animal cell cultures for production of recombinant proteins, because of features such as high safety, low cost, post-translation modifications and high volume of production. In this report, recombinant cDNA of tissue Plasminogen activator was transformed to tobacco plants. This gene was expressed under the control of CaMV35S promoter and NOS terminator. Kozak sequence and KDEL signal were linked at amino and carboxy-termini of tPA gene, respectively. The constructed cassette pBl(tPA) was transferred to agrobacterium and the tPA gene was inserted into the plant genome by agrobac terium-mediated transformation. Transgenic plants were grown on selective medium an* then transferred into perlite and soil, to obtain subsequent generation(To). The trans^ enic plants were tested by polymerase chain reaction (PCR), RT-PCR and Western blotting.