توسعه محيط كشت براي توليد لوواستاتين بوسيله آسپرژيلوس تريوس

Media Development for Production of Lovastatin j by Aspergillus Terreus

Production of lovastatin and microbial biomass by Aspergillus terreus were influenced based on the carbon and nitrogen sources by Aspergillus terreus were investigated. In this work, Taguchi experimental design was used to investigate the effect of C, N, oxygen content and fermentation time on the concentration of biomass and lovastatin production in batch process. The values of the various factors in the experiment ranged widely, as follows: 24-48 g/L C-concentration; 0.23-0.46 g/L N-concentration; 40-80% (v/v) oxygen and 11-14 days fermentation time. According to optimum conditions 48g/L lactose and 0.23 g/L soybean meal, 30 ±5 mg/L lovastatin produced during 11 days. Moreover 80% of maximum lovastatin production was obtained after 7 days of incubation.