اثر لوزارتان بر فيبروز كليه متعاقب انسداد كامل يكطرفه ميزناي در موش صحرايي

Effect of losartan on renal fibrosis after unilateral ureteral obstruction in rat

Losartan (LOS) is angiotensin receptor (ATI) antagonist and is used for treatment of congestive heart failure and hypertension. It is widely recognized that losartan has an organ protective nature and is very effective for organ damage progressing. Obstructive uropathy can be used to indicate any obstruction of urinary flow; which causes a developing of hydronephrosis, tubular atrophy, and associated renal impairment. The aim of this study was to evaluate the effect of losartan on renal fibrosis after unilateral ureteral obstruction in rat. In this experimental study, 50 Adult male Sprague-Dawley rats were subjected to unilateral ureteral obstruction (UUO) and randomly divided into five groups (ten rats in each groups) as follows: (1) control group; (2) UUO; (3) UUO/LOS; (4) Sham-operated; (5) Sham/LOS. Control animals received orally drug solvent by gavage for 15 days. Unilateral ureteral obstruction was performed in groups 2 and 3 and sham operations were performed in groups 4 and 5. In group 2, animals received drug solvent and in group 3, animals received losartan (60 mg/kg) for 15 days (start before the operation). Rats were sacrificed at day 14 for histopathological evaluation with hemathocilin & eosine, trichrome-masson and periodic acid-schiff technique. In this investigation, histopathologic evaluation approved that in UUO groups, renal interstitial fibrosis, tubular epithelial necrosis, hemorrhage, interstitial infiltration of mononuclear cells, tubular atrophy, glumerular tufts expanding, periglomerular sclerosis, subcapsular fibrosis, glomerulosclerosis, and peritubular capillaries edema were observed. But in losaratan treated animals, this histopatologic lesions and fibrosis were decreased. There were no differences between control and sham groups. The results showed that ureteral obstruction increased renal fibrosis and caused sever deterioration in renal tissue but losartan administration improved renal fibrosis. It needs more investigation to approve organ protective action of losartan in human renal disorders. Vet.J.of Islamic.Azad. Univ., Garmsar Branch. 4,4:187-195,2008.