مطالعه كاريولوژيكي چهار گونه Anthemis از ايران

Karyological study of four Anthemis species from Iran

In this study, we investigated karyological characteristics variation of four Anthemis species selected from various locations of Iran. We used of a-bromonaphthalene for pretreatment, Lewitsky solution for fixation, IN NaOH for hydrolysis and finally, aceto iron-hematoxylin for staining stage. Detailed karyotype features, i.e. total chromosome length (TL), total form percent (TF %), asymmetry index (AI), intrachromosomal asymmetry (Ai) and interchromosomal asymmetry (A2) were also estimated. Somatic chromosome number were 2n=18 for all species. Karyotypic analysis showed a predominance of metacentric chromosomes and satellite was observed in the shape of microsatellite in almost all of them. The largest chromosome and genome length belong to A. altissima. In addition, the four studied species showed varying interachromosomal (Ai; range = 0.28-0.40), and interchromosomal (A2; range = 0.12-0.15) asymmetry indices. According to Stebbins categories, they were separated to 2A (A. altissima, A. cotula & A. hyalina) and 1A (A. pseudocotula) karyotypic symmetry classes. A. pseudocotula had more symmetric karyotype than other species. Clustering of the Anthemis species and accesions based on cytogenetic data showed close relationship between A. cotula and A. hyaline. Chromosome count of A. hyaline and A. pseudocotula are reported here for the first time. Other two counts are agreement with the previous reports.