ريزازديادي گياه دم گاوي (Smirnovia turkestana Bunge)

Micropropagation of Smirnovia turkestana

Smirnovia turkestana is an endemic plant species of sandy deserts of Iran. This shrub and psamophyte plant belongs to the Papilionaceae family. It is an important species for protecting sandy soil, forage production and medicinal value. The aim of micropropagation of S. turkestana is for using it for desertification, overcome on its propagation, cryopreservation and .... Seeds were collected from sandy dunes of Kashan, Isfahan province. Seeds were scarified and sterilized by NaOCl (1.25% v/v) for 15 min; washed in distilled water and cultured on MS (half sucrose) medium. Shoots were isolated after 3 weeks and cultured on 8 medium treatments. The best treatment for shooting was MS basal medium supplemented with BA (0.5 mg/1), 2ip (0.5 mg/1) and IBA (0.01 mg/1). The best treatment for rooting achieved in modified MS medium without hormone. Rooted plantlets were transferred to pots containing field soil and grew well under greenhouse conditions.