بررسي تنوع ژنتيكي درختان آزاد با استفاده از ايزوآنزيم پراكسيداز برگ در سه رويشگاه جلگه اي شمال ايران

Genetic variation investigation on Zelkova carpinifolia, from three Iranian north lowland habitats using leaf peroxidase

In order to investigate genetic diversity in Zelkova carpinifolia population, sampling of leaves of 30 trees in three lowland habitats were done. For evaluating enzyme activities in the leaves, the samples extracted immediately and peroxidase studied quantitatively and qualitatively. Quantitative studies accomplished by spectrophotometer (in 530 NM wave lengths) and qualitative studies performed by polyacrilamide gel electrophoresis (PAGE). Quantitative activity analyzed with ANOVA method and showed no significant differences in the three habitats, but Mazandaran habitat showed the most average quantitative activity. Grouping of isoenzyme bands showed three ecotypes in three habitats. All trees of the habitats were classified into 6 groups using Ward method of cluster analysis on quantitative data. Assessing genetic distance showed the most distance between Golestan habitat individuals and the least genetic distance in Guilan habitat. Mazandaran and Guilan habitats exhibited low level of isoenzyme pattern relative to Golestan habitat. Golestan habitat had the most within group diversity and is suitable for Zelkova carpinifolia development. Result emphasized on utilizing of the most effective approaches for conservation of genetic biodiversity of the species.