بررسي الگوي تظاهر ژن هاي مربوط به چرخه سلولي در آرابيدوپسيس

Expression patterns of cell cycle-related genes in Arabidopsis thaliana

Cell cycle, which is the basic reference for cell division and growth, is precisely regulated. Control of cell cycle progression in animal and plants needs regulation of gene expression. In this study, by means of inducing synchronization of Arabidopsis suspension cells using aphidicolin, trend of expression changes in several genes involving in different stages and biochemical pathways of cell cycle was investigated. Application of highly sensitive detection method, qRT-PCR enabled us to precisely monitor the behavior of the genes in different stages of the cell cycle. Expression patterns of 69 core cell cycle-related genes and 81 transcription factors were compared, and their possible roles in the specific stages of the cell cycle were analysed. Among the genes showing differential expression during progression of the cell cycle, cyclin type A and B and CDK have been reported to play key roles. The expression patterns of most of cyclin type A and B genes appeared to be similar. A subset of the cyclins, CYCA3 showed maximum expression in S phase which is considered as the most important kinases involved in this phase. CDK genes showed important role in Gl/S and G2/M transition stages. Many transcription factors belonging to different transcription factor families showed fluctuations in different time of progression of the cell cycle that play significant role in the precise control of the cell cycle processes.