ارزيابي ميزان فنل تام و فعاليت هاي آنتي اكسيداني بومادران، درمنه و بابونه

The Antioxidant Activities and Total Phenolic of Artemisia Martima, Achillea Millefolium and Matricaria Recutica

Introduction & Objective: Consumption of plant derived antioxidant contributes to reducing risks of certain chronic and degenerative diseases. The aim of the present study was to study the antioxidant activities and total phenolic of Artemisia Martima, Achillea Millefolium and Matricaria Recutica Materials & Methods: The present study was conducted at Yasuj University of Medical Sciences in 2009. The Stem and flower sample of plants were air-dried, and then grinded and were finally extracted by ethanol: water (70: 30) for 48 h in room temperature. Extracts were filtered and dried under vacuum system. The antioxidant activity of three ethanol extract of the medicinal plants, Artemisia martima, Achillea millefolium and Matricaria recutica, were analyzed by five different methods; (1,1-diphenyl-2- picrylhydrazyl (DPPH) radical, 2, 20azinobis- (3- ethylbenzthiazoline -6-sulphonic acid (ABTS) radical cation,Ferric- reducing antioxidant power assay (FRAP), phosphomolybdenum (PMB) and reducing power ( RP). In addition, for determination of antioxidant components, the total phenolic content was also analyzed. The collected data was analyzed by SPSS software. Results: For all antioxidant activity assays, Artemisia martima had the highest antioxidant activity value and also total phenol content. Antioxidant capacity analyses revealed that the FRAP and DPPH had comparable results. Antioxidant activity at 1 mg/mL, in ABTS were in the order Artemisia martima> Achillea millefolium> Matricaria recutica. Similar trend was observed for PMB content. RP, FRAP and DPPH were in the order Artemisia martima> Matricaria recutica > Achillea millefolium . Conclusion: The extracts showed a variety of antioxidant activities in all antioxidant assay system. This study demonstrated that Artemisia martima crude extract exhibit significant antioxidant activity. Key words: Antioxidant Activity, Total Phenol, Total Flavonoids, Medicinal Herbs