تعيين ميزان حساسيت ايزوله هاي كانديدا نسبت به داروي ايتراكونازول با مقايسه روش هاي ديسك ديفيوژن و رقيق سازي در مايع

Correlation between disk diffusion and broth micro dilution methods for determining susceptibilities of Candida spp. to Itraconazole

Increasing prevalence of Candidasis with the emergence of non-albicans Candida spp. and acquiring antifungal resistance has showed routine antifungal susceptibility testing. In present work, Itraconazole(ITZ)susceptibility of 106 Candida strains of 8 different species isolated from clinical specimens of patient were studied by standard disk diffusion method(D.D) and the result were compared with results obtained form the broth micro dilution-based reference method(B.M.D).Susceptibility ranking to ITZ obtained with all yeasts tested was decreased from C.dubliniensis to C.parapsilosis, C.lusittani, C.kefyr, C.albicans, C.tropicalis, C.glabera and C.krusei. C.krusei and C.glaberata showed the highest rate (5.66%/7.54%) of S-DD/R among all species tested. In D.D and B.M.D method 85.85% and 86.79% of isolates were susceptible(S), whereas 10.37% and 7.54% showed dose-dependent susceptibility (S-DD), and 3.77% and 5.66% were resistant(R), respectively. A total correlation between D.D & reference methods was measured as 89.1 %(p<0.05).this data suggest The D,D method is a rapid, simple and reliable screening test for routine susceptibility testing use.