مقايسه روش هاي مختلف استخراج اسيدگليسريزيك از پودر عصاره شيرين بيان

Comparison of Different Methods of Obtaining Glycyrrhizic Acid from Licorice Extract Powder

Academic Member of the Department of Organic Chemistry, Science and Research Branch, ^ Islamic Azad University, Tehran, Iran. g 06 d Academic Member of the Department of Plant breeding, Science and Research Branch, z o Islamic Azad University, Tehran, Iran. e 1 Agriculture Engineering-Food Science and Technology Expert, Tehran Pars Minoo Factory. Received: 12 August 2009 Accepted:21 January 2010 Abstract Introduction: Licorice as a native and traditional plant of Iran contains Glycyrrhizic acid » (GA) or Glycyrrhizin, a substance claimed to be up to 30-50 times sweeter than sucrose. £ Three different techniques of glycyrrhizic acid extraction from licorice extract powder have been compared. Materials and Methods: Modified Roussin technique (Method 1), Dissolving the licorice ^ extract powder in distilled water and treatment with ethanol and sulphuric acid (method 2) . 1 Application of microwave (Method 3). High performance liquid chromatography was used 9 to separate and determine GA and find the yield of extraction by various techniques. c- Results: The results showed that the yield by the third method of extraction was higher ^ (28.84%) than the second (8.5%) and the first method (4.6%),which might be due to the / effect of higher temperature and pressure applied in microwave technique. •£. Conclusion: Microwave extraction method proved to be a quicker method and provided £ higher extraction yield as compared to other conventional methods. 1 Keywords: Extraction Techniques, Extraction Yield, Glyc