شناسايي مولكولي فسفوليپاز3Bبعنوان عامل موثر در بيماريزايي آسپرژيلوس فوميگاتوس

Molecular Detection of Phospholipase B3 as an Effective Factor in Virulence of Aspergillus Fumigatus

Phospholipases are the major component of all eukaryotic bilayer cell membranes, and serve as a scaffold for membranes proteins. Phospholipases are a class of ubiquitous enzymes that have estrase activity and due to their ability to lyses of eel! membrane can play aii important role for pathogenicity of mierorganisims. During this study using degenerate primers based on homologous amino acid sequences of sonic fimg? phospholipase B (PI B), detection of AspergiHus jiimigatu: PLB? \v- > earner OI.;l Maiencrfs and Methods Alter DM/,, extraction of , venonatum and Aumnasiahtn. out using degenerate primers. fumigatus was cloned in pGEVtT-Easv vecto. and then transformer: into E. coli Top !(■ r competent eel! for t^xinction of cloned DNA fragment. Result Sequence analysis of 2100 bp fragments using .BLASTX software were revealed a high homology to published PLB sequences from other fungi. Conclusion jumigutus, A, mgei\ Pus an wiiiuians PC i . reaction was can Predicted 21bp product from Keywords: Aspergillus fumigates, Phospholipase B3, Virulence Phylogenic tree analysis of PLB3 gene shows that PLB and potential PLB analogues like lysophospholipase are contained in a large cluster of the PLB family. A. fumigatus PLB gene is more closely related to A. oryzae, A. niger, Penicillium chrysogenum and Neurospora crassa than other fungi. Also, sequence availability of full length PLB3 gene represents a noteworthy breakthrough in the study of this opportunistic pathogen and function of PLB3 gene in pathogenesis of A. fumigates. Detection of PLB3 gene can be lead to preparing vaccine, blocker and laboratory marker for recognition of PLB3 gene products either in patient body or clinical samples.