روش ايمونوهيستوشيمي در تشخيص لنفوم هوجكين و مقايسه آن با روش هاي متداول

Comparative Immunohisto chemical Diagnosis of Hodgkinʹs lymphoma with Conventional Method

Introduction Hodgkinʹs lymphoma is a malignant neoplasm of B lymphocytes and less common T lymphocytes that morphologically can be mistaken with several benign and malignant diseases. These diseases have different course, treatments and prognosis from Hodgkinʹs lymphoma. Immunohistochemical staining is very useful that will aid in separating Hodgkinʹs Lymphoma from a series of benign and malignant lookalikes. Materials and Methods We Randomly selected 53 cases with primary diagnosis of Hodgkinʹs lymphoma in. pathology laboratory of Imam Reza Hospital from 1374-1384. to confirm or rule out of the primary diagnosis, microscopic evaluation and Immunohistochemical staining were done. Results Overall in 47 cases (88.7%), Hodgkinʹs lymphoma was confirmed and 6 cases (11.3%) were non hodgkin lymphoma.A total 5 cases were T cell Rich large B cell lymphoma and 1 case was anaplasic large cell lymphoma probably. In Hodgkinʹs lymphoma cases, the mean age was 40 years. Except for nodular sclerosis other types were more frequent in males. (M/F = 2.5).The commonest type was mixed cellularity (39 cases) (54.7%). Conclusion Misdiagnosis of Hodgkinʹs lymphoma without Immunohistochemical staining was 11.3%. Because of significant difference between treatment and prognosis of Hodgkinʹs lymphoma and other morphologically similar diseases, immunohistochemical staining particulary in lymphocytic depletion and nodular lymphocytic predominant Hodgkinʹs lymphoma, is necessary and recommended. Keywords: Hodgkinʹ s Lymphoma, Immunohi stochemi stry, Lymphoma,