بررسي ژنوتيپ هاي ويروس سينسيشيال تنفسي انسان در كودكان زير 5 سال مبتلا به عفونت حاد تنفسي در سال هاي 1386 و 1388

A Study of Human Respiratory Syncytial Virus Genotypes in Children Less Than 5 Years of Age with Acute Respiratory Symptoms in 2007 and 2009

Introduction: Human respiratory syncytial virus (HRSV) is the most important viral agent of acute respiratory infection in children less than 5 years age worldwide. HRSV is responsible for 50% brochiolitis and 25% pneumonia in infants. There are limited data of HRSV genotypes from developing countries. The aim of this study was to investigate HRSV genotypes in 2007 and 2009 in Iran. Materials & Methods: 72 and 107 respiratory samples were collected from children less than 5 years of age with acute respiratory infection in 2007and 2009, respectively, whom were tested for G glycoprotein gene of HRSV by RT-PCR. Findings: Of the 72 respiratory samples tested in 2007, 14 samples (19,44%) were positive for HRSV and fell on three genotypes: 12 strains in GA2, 1 strain in GA1 , and 1 strain in GAS. Whereas, of the 107 respiratory samples tested in 2009, 24 samples (22,24%) were positive that 16 (67%) belonged to subgroup A genotypes: 15 strains in GA1, and 1 Strain in GA2, whereas, 8 (33%) clustered in genotype BA in subgroup B. Discussion & Conclusion: This study revealed that multiple genotypes of HRSV were cocirculated on years 2007 and 2009 in Iran. Also our results showed that subgroup A strains were more prevalent than subgroup B viruses, GA2 and GA1 genotypes were predominant genotypes on years 2007 and 2009, respectively. Keywords: human respiratory syncytial virus, genotype, acute respiratory infection tropical and developing countries .Trop Med Int Health 1998; 3: 268-280. 18- Choi EH, Lee HJ. Genetic diversity and molecular epidemiology of the G protein of subgroups A and B of respiratory syncytial viruses isolated over 9 consecutive epidemics in Korea. J Infect Dis 2000; 18: 1547-56. 19- Parveen S, Sullender WM, Fowler K, Lefkowitz EJ, Kapoor SK, Broor S. Genetic variability in the G protein gene of group A and B respiratory syncytial viruses from India. J Clin Microbiol 2006; 44: 3055-64. 20- Galiano MC, Palomo C, Videla CM, Arbiza J, Melero JA, Carballal G. Genetic and antigenic variability of human respiratory syncytial virus (groups a and b) isolated over seven consecutive seasons in Argentina (1995 to 2001). J Clin Microbiol 2005; 43: 2266-73. 21- Zlateva KT, Lemey P, Moes E, Vandamme AM, Van Ranst M. Genetic variability and molecular evolution of the human respiratory syncytial virus subgroup B attachment G protein. J Virol 2005; 79: 9157-67. 22- Venter M, Madhi SA, Tiemessen CT, Schoub BD. Genetic diversity and molecular epidemiology of respiratory syncytial virus over four consecutive seasons in South Africa: identification of new subgroup A and B genotypes. J Gen Virol 2001; 82: 2117-24. 23- Ostlund MR, Lindell AT, Stenler S, Riedel HM, Wirgart BZ, Grillner L. Molecular epidemiology and genetic variability of respiratory syncytial virus (RSV) in Stockholm, 2002-2003.J Med Virol 2008; 80(1): 159-67. 24- Chan PW, Chew FT, Tan TN, Chua KB, Hooi PS. Seasonal variation in respiratory syncytial virus chest infection in the tropics. Pediatr Pulmonol 2002; 34: 47-51. 25- Weigl JA, Puppe W, Schmitt HJ. Seasonality of respiratory syncytial virus- positive hospitalizations in children in Kiel, Germany, over a 7-year period. Infection 2002; 30: 186-92. 26- Bdour S. Respiratory syncytial virus subgroup A in hospitalized children in Zarqa, Jordan. Ann Trop Paediatr 2001; 21: 253-61. 27- Resch B, Gusenleitner W, Muller W. The impact of respiratory syncytial virus infection: a prospective study in hospitalised infants younger than 2 years. Infection 2002; 30: 193-7. 28- Milani M .[Respiratory syncytial virus infection among young children with acute respiratory infection] .Acta Medica Iranica 2003; 41(4): 269-72. (Persian) 29- Hal 1 CB, Walsh EE, Schnabel KC, Long CE, McConnochie KM, Hildreth SW, et al. Occurrence of groups A and B of respiratory syncytial virus over 15 years: associated epidemiologic and clinical characteristics in hospitalized and ambulatory children. J Infect Dis 1990; 162: 1283