Optimization of Dispersive Liquid - Liquid Microextraction of Water_Soluble Components of Rosa Damascena Mill . Essential Oil by Experimental Design

I n the present work, dispersive liquid-liquid microextraction (DLLME) was appl ied for the. recovery of water-soluble constituents of Rose essential oil. In this experiment analytes insedimented phase were determined by GC-MS. Some important parameters of DLLME suchas volume of extractant and disperser sol vents, temperature and salt effect were inspected bya full factorial desi gn to identify important parameters and their interactions . This exploratorydesign showed that salt addition has no effect on the efficiency. Then, a response surfacemethod was applied to obtain optimum point of the first three parameters . The optimal conditionwas obtained as 37.0 ~IL of extractant, 0.42 mL of disperser and temperature of 48 °C. Themain components that were extracted from Rose water by this method were: benzeneethanol(25.15%), geraniol (23 .07%), beta-citronellol (22 .38%), nerol (8.48%), eugenol (5.98%) andlinalool (5 .62 %).