مطالعه تجربي آپوپتوز در لوزالمعده جوجه هاي SPF متعاقب عفونت با ويروس آنفلوانزاي سروتيپ H9N2

Experimental study of apoptosis in pancreas of SPF chickens following infection by H9N2 serotype of influenza virus

Influenza virus is the cause of cell death in animal and human tissues. Cell death can take place in the form of necrosis or apoptosis. In this study, the type of cell death in the pancreatic tissue of chickens infected by H9N2 serotype of influenza virus (A/chicken/Iran/772/2000) is experimentally investigated. In the present study, 20 SPF chickens with the age of 3- weeks were randomly allocated to two equal groups. The first group was infected with 0.2 ml of 1:10 dilution and 107. 5 EID50 titer of the virus and the second group with equal volume of normal saline solution intranasaly. After 72 hours, specimens were collected from the pancreatic tissue and 5 Mm thick microscopic sections were prepared from specimens fixed in 10% buffered formalin and stained with hematoxylin and eosin. Histopathological examination of the pancreatic tissue revealed that there was significant difference in the mean number of apoptotic and necrotic cells between the control and treatment groups (p<0.005). The results of this study indicated that the H9N2 serotype of influenza virus has the potential to induce apoptosis in acinar, alpha and beta islet cells of pancreas.