كالوس زائي و اندام زائي گياه داوودي Chrysanthemum morifolium ramat L

Callogenesis and organogenesis of Chrysanthemum morifolium Ramat L

The effects of different cytokinins (benzyl amino purine(BAP), Kinetine (Kin) in combination with different auxins( Indole butyric acid(IBA), 2,-dichlorophenoxy acetic acid (2,4-D) were investigated on callogenesis and organogenesis of Chrysanthemum morifolium Ramat L. Leaves explants were cultured in the MS medium supplemented with different auxins and cytokinins. Results of using IBA and BAP in the media indicated that calli which were taken from medium with IBA (2 mgL-1) and BAP (0.5 mgL-1) produced roots and callus of IBA (1 mgL-1) and BAP (lmgL-1) produced shoots. By using 2,4-D and Kin in the medium, rhizogenesis were occurred in the media supplemented with 2,4-D (0.5 ,4 mgL-1) and Kin (2 mgL-1).Subculture of calli increased rooting. Calli of medium with 2,4-D (0.5 mgL-1) and Kin (2mgL-1) produced shoot after the first subculture in the free hormone medium. In the 2,4-D and BAP treatments, maximum level of callogenesis and rhizogenesis were achieved. Calli in the medium supplemented with 2,4-D (0.5 mgL-1) and BAP (lmgL-1) produced roots and shoots.