بررسى اثر آسكوربيك اسيد (ويتامين C) بر رشد و نمو جوانه اندام حركتى جنين موش تحت شرايط in vitro

Effect of ascorbic acid (vitamin C) on the development of mouse limb-buds in vitro

Ascorbic acid (vitamin C ) is one of the water soluble vitamins. This vitamin is synthesized from glucose in the liver in some mammals. This process is not seen in primates and the guinea pigs. Vitamin C is an important antioxidant and plays an essential role in the synthesis of certain extracellular matrix proteins. In this experimental study we examined the effects of vitamin C on the limb-bud development in vitro using NMRI mouse strain as an Animal model. On day 11.5, pregnant mice were killed and the embryosʹ limb-buds were dissected and grouped as control, expl & 2. A sample from each group was cultured using the Trowell technique in vitro. The different doses of ascorbic acid used in these experiments were 50Mg /ml & l00Mg /ml for expl & 2, respectively. Culture dishes in control samples contained only Eagles Minimum Essential Medium (EMEM), supplemented with 15% human embryonic cord serum and antibiotics. Samples from the three groups were incubated in 37°C, 5% C02 and 95% air condition. Morphological and histological studies in control. expl& 2 groups showed a dose- related increase in length growth along the proximo-distal axis in expl & 2 with respect to the control group. Number of mesenchymal cells in the experimental groups decreased significantly in vitro; this occurs due to the differentiation of mesenchymal cells to chondrocytes. Results also showed a significant increase in the number of red blood cells in the experimental groups. Number of chondrocytes also increased significantly in the experimental groups. Especially in the proximal zone, hypertrophic chondrocytes were observed in the experimental groups. The results of this study demonstrated that the antioxi dative, proliferative and differentiating effects of vitamin C act in a dose dependent manner.