عنوان مقاله :
مقايسه بيومس توليد شده بروسلا آبورتوس در فرمانتور به روش كشت بسته (Batch) و فد-بچ (Fed-batch)
عنوان به زبان ديگر :
Conjugation of Detoxified Lipopolysaccharide and O-polysaccharide from Brucella abortus with Tetanus Toxoid to prepare brucellosis Vaccine
پديد آورندگان :
شاپوري ، رضا نويسنده Shapouri, R , زواران حسيني ، احمد نويسنده Z. Hosseni, A , حسيني دوست، رضا نويسنده گروه ميكروب شناسي و مركز تحقيقات بيولوژي مولكولي- دانشگاه علوم پزشكي بقيه ا...(عج)- تهران- ايران hosseini doust, reza , احمدي، حجت نويسنده پرديس كشاورزي ومنابع طبيعي-دانشگاه تهران ahmadi, hojat , تبرايي، بهمن نويسنده Tabaraie, B , محبتي مبارز، اشرف نويسنده M. Mobarez, A
اطلاعات موجودي :
فصلنامه سال 1386
كليدواژه :
بروسلا آبورتوس , بيومس , فرمانتور , كشت فد-بچ , كشت بسته
چكيده لاتين :
Brucellosis is a zoonotic disease that causes heavy economic losses and human morbidity. The goal of this study was to prepare a subcellular vaccine for brucellosis. Detoxified Lipopolysaccharide (LPS) and O-polysaccharide (OPS) from B.abortus S99 were conjugated with tetanus toxoid (TT) as a carrier protein. The LPS was extracted by the hot phenol procedure and detoxified. The OPS was extracted using acetic acid. D-LPS-TT and OPS-TT conjugates were prepared by amidation method. The endotoxin activity assay showed 0.125 (EU)/Mg of D-LPS and OPS that were acceptable to use for immunization. The average PS/protein ratios were 0.4 and 0.33 for D-LPS-TT and OPS-TT respectively. The labeling efficiencies were 39 and 44% for D-LPS-TT and OPS-TT respectively. The double immunodiffusion indicated that the conjugates reacted with a single line of identity against the standard Brucella antiserum and tetanus antitoxin. The rabbit thermal induction showed no excess in body temperature with 10Mg of the conjugates injections.
عنوان نشريه :
فيزيولوژي و تكوين جانوري
عنوان نشريه :
فيزيولوژي و تكوين جانوري
اطلاعات موجودي :
فصلنامه با شماره پیاپی سال 1386
كلمات كليدي :
#تست#آزمون###امتحان
