عنوان مقاله :
استفاده از نشانگرهاي ريز ماهواره پيوسته با صفت مقاومت به ويروس IHN در غربالگري جمعيت مولدين ماهي قزل آلاي رنگين كمان (Oncorhynchus mykiss)
عنوان به زبان ديگر :
Use of microsatellite associated with resistance to IHN virus in screening of Rainbow trout brood stock population
0Oncorhynchus mykiss)
پديد آورندگان :
محمد طاهري، مژده نويسنده دانشگاه تهران,دانشكده منابع طبيعي Mohammadtaheri, mojdeh , قوتي، ندا نويسنده دانشگاه تهران,دانشكده منابع طبيعي ghovati, neda , مجازي اميري ، باقر نويسنده Mojʹazi Amiri, bagher , فرحمند، حميد نويسنده Farahmand, hamid , ميرواقفي، عليرضا نويسنده دانشگاه تهران, Mirvaghefi, alireza , خليلي، بيتا نويسنده دانشگاه تهران,دانشكده منابع طبيعي Khalili, B
اطلاعات موجودي :
فصلنامه سال 1389
كليدواژه :
قزل آلاي رنگين كمان , مقاومت به بيماري IHN , نشانگرهاي ريز ماهواره , انتخاب مبتني بر نشانگرها , غربالگري ژنتيكي
چكيده لاتين :
The aim of present study was to screen Rainbow trout broodstock population of Kelardasht Reproduction and Rearing Center, with emphasis on presence and non-presence of Microsatellite markers associated with resistance to IHN virus. For this purpose, 92 samples of caudal fin tissue from Kelardasht Rainbow trout brood stock population were obtained and genomic DNA of these samples were extracted. Two microsatellite loci include Ssa7NVH, Ssa94NVH were amplified using the polymerase chain reaction (PCR) with their specific primers. This study showed that presence of these microsatellite markers in this brood stock population were 98.49%, 95.46% and the number of polymorphs for those were 4, 11 respectively. According to sex-differentiation, the presence of these microsatellite markers based on Ssa94NVH in male and female were 92.4 & 97.5 and based on Ssa7VNH were 100 & 97.5 respectively.
^Corresponding author:
Keywords: Rainbow trout, microsatellite, resistance to IHN virus, genetic screening, marker assisted selection (MAS)
اطلاعات موجودي :
فصلنامه با شماره پیاپی سال 1389
كلمات كليدي :
#تست#آزمون###امتحان
