رديابي پاسخ پادگني پروتيين H ويروس طاعون گاوي

Tracking of antigen response hemagglutinin protein (H) of rinderpest virus

طاعون گاوي Rinderpest يكي از بيماري هاي حاد و گاهي فوق حاد و خيلي مسري گاو و عده اي از نشخوار كنندگان بوده كه از دير باز شناخته شده است. ويروس طاعون گاوي داراي دو گليكو پروتيين سطحي H وF است كه آنتي بادي‌هاي خنثي كننده را القا مي‌كنند. پس از خالص‌سازي ويروس و جداسازي پروتيين‌هاي سطحي ويروس (با استفاده از محلولTriton X-114 ) ميزان پروتيين موجود با استفاده از روش پروتيين‌سنجي لوري اندازه‌گيري شد و پس از انجام الكتروفورز عمل انتقال پروتيين‌هاي حاصله بر روي كاغذ نيتروسلولز به روش وسترن بلات بدست آمد تا از ويژگي آنتي بادي مونوكلونال 4B4L1C6 (عليه پروتيين H ويروس طاعون گاوي) اطمينان حاصل شود.

Rinderpest (RP), popularly known as cattle plague, is one of the oldest known acute and fatal viral disease of domestic livestock like cattle. Rinderpest virus (RPV), causative agent of rinderpest disease, was purified and concentration of its protein determined by lowry assay, and the concentration of RPV protein solution and separated surface proteins was determined 1536 and 329.5 µg/ml respectively. Hemagglutinin (H) protein of RPV separated by using Triton x-114 detergent. The H protein had an apparent molecular mass of 69 KD as measured by SDS-PAGE, with respect to molecular weight marker, at the end of, Immunogenenicity of this protein determined by SDS-PAGE and western immunoblotting by specific mAb for it. Immunogenic properties of recombinant baculovirus expressing the membrane-bound H protein have been shown, but separation of H protein of RPV by using detergent and investigation of its immunogenicity are novel works completely.