تاثير تجويز خوراكي سيلي مارين بر روي فاكتورهاي بيوشيميايي خون قزال آلاي رنگين كمان(Oncorhynchus mykiss)

Effects of oral administration of silymarin on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss)

Silymarin, the extract of Milk thistle seeds (Silybum marianum L.) is used to treatment diseases such as diabetes, hepatitis, cancers, cirrhosis and liver failure, drug and toxic poisoning, etc. This herbal medicine as a food supplement may be useful in treatment of different disease of animals including fish. However, it is necessary to assess the clinical effects of a drug on health status of animals before administration. This study thus aimed to assess oral administration of 0, 100, 400, 800 mg silymarin per 1 kg of commercial food on blood biochemical of rainbow trout in the laboratory. Oral administration of silymarin reduced significantly (P < 0.05) plasma glucose and cholesterol levels and increases relatively plasma total protein and globulin concentrations. Increase of plasma albumin levels reflect the important role of albumin at drug transportation in circulatory system of fish. Silymarin also stabilizes cellular membrane structure and regulates levels of aspartate aminotransferase (AST), alanin aminotransferase (ALT), alkaline phosphatase (ALP), creatinin phospokinase (CK) and lactate dehydrogenase (LDH) activity. In conclusion,, oral administration of silymarin, up to 400 mg/kg of food, have no side effect on blood biochemical and clinical parameters of fish. However, oral administration of 800 mg/kg silymarin resulted in cytotoxicity and alternation of blood biochemical parameters in rainbow trout.