تاثير محلول هاي فعال كننده نمكي اسپرم بر موفقيت لقاح قزل آلاي رنگين كمان (Oncorhynchus mykiss)

Effect of sperm activating solutionon improvement of reproduction efficiency of rainbow trout (Oncorhynchus my kiss)

This research has been conducted at the end of propagation season (April) at the Salmonid reproduction and conservation center of Shahid Bahonar-Kelardasht in late 2008. For this order the effect of 4 different saline activator solutions on fertilization rate, eyed eggs and hatching stage were investigated in comparison to water in the reproduction center. After preparing different diluents, 15 males and 10 females were striped. For the measurement of sperm motility, we used light microscope and this period was measured until 95-99% of the sperm cells did not have any mobility. There were significant differences at levels of 95% among different saline activator solutions with the water of propagation center on sperm motility, fertilization, eyed eggs and hatching stage. Results showed that using activator solution with component of NaCl, 7/305 gr/L; CaC12.2H20, 0/735 gr/L; pH: 7/7 had the highest fertilization (61.22±3.26%), eyed eggs (57.48±3%) and hatching stage (95/18±./57%). The noel groups had the lowest fertilization, eyed eggs and hatching stage in comparison of others group.