بررسي توليد مواد فعال زيستي از لحاظ بيولوژيك (Bioactive Compounds) در باكتري‏هاي ايزوله شده از آب‏هاي ساحلي درياچه كاسپين

Evaluation of Bioactive Compounds produced by bacteria isolated from coastal region of Caspian Sea

The aim of these research have been isolation and recognization of bacteria from costal region of Caspian Sea and evaluation their potency for bioactive compounds with anti bacterial activity production. In this study, at first 29 water samples were collected from costal region of Caspian Sea and analyzed for isolation the bacteria. After culture and purification the samples, the ability of bioactive compounds with anti bacterial production of the isolated bacteria were assessed by agar well diffusion method against 10 human pathogen, and then the isolated bacteria that produced these compounds were characterized based on biochemical methods on Bergys Manual of Systematic Bacteriologi, 16Sr RNA gene sequence analysis had done on three of them with wide spectrum effect. Then hemolytic activity of these isolated compound were examined. A total, 108 bacteria were isolated and amongst these, 12 bacteria showed anti bacterial activity against at least 3 strains of 10 strains of human pathogen. Biochemical characterization of these bacteria showed that these bacteria were mainly Pseudomonas sp. and Bacillus sp. The phylogenetic analysis using 16Sr RNA sequencing on three strain releaved that 100% affiliation with Bacillus cereus and 99/7% with Alcaligenes faecalis and 99/7% with Exiguobacterium acetylicum strain DSM 20416. The most antibacterial activity abserved in Pseudomonas aeroginosa that showed antibacterial activity in 10 strain of human pathogens.