شناسايي مولكولي subsp. Paratuberculosis Mycobacterium avium در گاوهاي شيري شهرستان تنكابن (شمال ايران)

Molecular identification of Mycobacterium Paratuberculosis isolated from dairy cattles in city Tonekabon (Northern Iran)

Recent study is the first attempt to identify infected dairy cattle with Mycobacterium paratuberculosis which is done with Johnin test and PCR method in Tonekabon. Paratuberculosis is a chronic intestinal infection in ruminants and spread in different ways to environment. Milk is important source of bacteria. Also, due to the possible relationship between Johneʹs disease and Crohnʹs disease in humans, presence of bacteria in milk can be an important index to assess the spread of infection in animal and human. In this study, at first, 90 dairy cattle were tested by Johnin test and result were as follow, 3 positive cases, 23 suspected cases and 62 negative cases. Then the samples examined using nested PCR, then two pairs of primers were used to increase of desired pieces of 194 bp were used. The prevalence results were different in three verify regions and showed desired ranges between 4.2 and 7.7%. As a result, tests revealed that Johnin test doesnʹt have an ability to identify suspected cases, then it is necessary to use PCR techinique to identify the exact animal patient.