بررسى اثر عصاره آبى و الكلى گياه كانابيس ساتيوا بر دانسيته نورونى منطقه سوبيكولوم هيپوكامپ در موش صحرايى نر

The effect of leaves aquatic and alkolic extract of Cannabis sativa on neuronal density of Hippocampus subiculum in male rats

Memory has many especial tract for various kind of input information. In Cannabis sativa was found more than sixty one substance that cannabinoid was named. The aim of this study is the evaluation of leaves aquatic and alkolic extract of Cannabis sativa effect on neuronal density of subiculum in male rats Fourty male Wistar Rats weight (300-350 g) were completely divided into four experimental groups and control group. Cannabis sativa was extracted with Soxhlet apparatus. Aquatic and alkolic extract was injected peritonealy (I.P.) in experimental groups with dosage (50 mg kg-1. 25 mg kg-1) for three week. After one month. Animal was decapitated and their brain dissected. fixed in 10% formalin. sectioned in 7Mm thickness and stained by H.E. By applying stereological techniques and systematic random sampling scheme the neuronal density of cubicu-lum were estimated. Statistical analyses showed significant indecrease (p<0/001) in the subiculum neuronal density in experimental groups((dosage 25.50 mg/kg Aquatic extract) in compare to control groups and showed significant increase (p<0/001) in the subiculum of Hippocampus neuronal density in experimental group in (dosage 25 mg/kg Alkolic) compare to control groups. Aquatic extraction of Cannabis sativa leaves that effectively block the release of k+ and induced neuronal degeneration in subiculum in way that reduced the neuronal density of subiculum. While, alkolic extract of this material induces a nurogenese.