مطالعه اثر آنتي اكسيداني هايپوتائورين در كاهش آسيب هاي ناشي از استرس اكسيداتيو در رشد جنين هاي موش سوري حاصل از لقاح آزمايشگاهي

ANTIOXIDATIVE EFFECT OF HYPOTAURIN ON SUPPRESSION OF OXIDATIVE TENSION-INDUCED DAMAGE ON THE DEVELOPMENT OF MOUSE EMBRYO FERTILIZED IN VITRO

Background &Aims: There are multiple mechanisms of embryo protection against ROS. However, in the in vitro condition these reagents are absent and the level of ROS is higher. This study was aimed to survey the morphological damage induced by oxidative stress and also to study the effect of Hypotaurin as an antioxidant on mouse preimplantation embryo development in two different conditions of normal and oxidative tension In vitro. Material & Methods: Following in vitro fertilization, the zygotes were cultured in different groups in HTF medium containing 4 mg/ml BSA. To survey the effect of oxidative stress on embryo development, zygotes were cultured for one hour in a medium containing different concentrations of H202 and were transferred to the culture after washing. In order to study the effect of Hypotaurin on embryo development in oxidative tension system, the zygotes were placed in a medium containing different concentrations of Hypotaurin after culturing the zygotes in 10 jiM H202 for 1 hour. Along with the effect of Hypotaurin on embryo development was surveyed by adding different concentrations of Hypotaurin to the culture without oxidative tension. Results: After short time exposure to H202, the preimplantation embryo development decreased significantly and this was more obvious in higher concentrations. Hypotaurin reduced the impairment resulting from oxidative tension slightly and improved the embryo development, quality, and the cleavage rate and decreased embryo arrest. It also improved the cultured embryos morphologically (p<0.05). Conclusion: To suppress the deleterious effects of ROS in embryo culture system various antioxidants can be employed. The present study showed that oxidative stress induced embryo arrest and dismoiphogenesis and reduced the embryo development. However, supplementation of the embryo culture with Hypotaurin improved the embryo. Therefore, adding antioxidants such as Hypotaurin to embryo culture system restores the embryo growth from damages. Keywords: ROS, Zygote, Embryo arrest, Hypotaurin, Mouse Original Artie The Journal of Urmia University of Medical Sciences, Vol. 21(4), Nov-Dec, 2010 Address: Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Tel: (+98) 9141498524- (+98) 4412770508