اثرات وابسته به دوز ملاتونين بر ظرفيت بلوغ آزمايشگاهي تخمك، لقاح آزمايشگاهي و تكوين بلاستوسيست در موش

Melatonin dose-dependent effects on oocyte maturation capacity, in vitro fertilization and blastocyst development in mouse

Aims: Considering that melatonin is known as an effective ROS scavenger, it may be able to improve the in- vitro oocyte maturation and the fetal quality. This study was designed to determine the effect of melatonin on in- vitro oocyte maturation and fertilization and cleavage and blastocyst development rate in mice. Materials & Methods: This experimental study was carried out in the cellular-molecular center of Gilan Medical University in 2010. The cumulus-oocyte complex (COC) and germinal vesicle were obtained from mice. COCs were cultured for 18 hours in maturation medium (supplemented with different doses of melatonin (100, 10, 1 |LiM, 100, 10]LiM) and control group and their maturation rate was assessed. Then two pre-nucleus embryos were cultured in T6 medium supplemented with different doses of melatonin and embryosʹ developmental rate was recorded until blastocyst stage by inverted microscope. Data were analyzed using Chi- square test by SPSS 13 software. Results: 1, 10 and 100 |iM concentrations of melatonin had positive effect on in-vitro oocyte maturation and fertilization in mice compared to the control group (p<0.01). In addition, the cleavage and blastocyst development rate increased in presence of lOand 100 nM of melatonin (p<0.01). Conclusion: Melatonin as an effective ROS scavenger has positive influence on in-vitro oocytes maturation and embryosʹ development promotion in mice.