مطالعه اثرات بازدارندگي داروي سديم سولفابنزاميد بر رشد و تكثير سلول هاي T-47D با تاكيد بر آپاپتوزيس و توقف چرخه ي سلولي

Study of the inhibitory effects of sodium sulfabenzamide on the proliferation of T-47D cells with emphasis on apoptosis and cell cycle arrest

In the line of widespread studies on sulfonamides, anti-tumoral effects have been reported for this drug family. Appearing of these anti-tumoral effects in sulfonamide drug family has been attributed to some cell and molecular mechanisms such as inhibition of membranous carbonic anhydrase, prevention of the formation of microtubules, arresting of cell cycle and prohibition of angiogenesis. In this research project, sodium sulfabenzamide drug with low inhibitory potency for carbonic anhydrase was selected until its net effect on some cellular processes such as apoptosis and cell cycle arrest to become able to be placed under study. Using MTT test, prevention concentration of drug for 50 percent reduction of the cells viability in the medium was obtained as 10.8 mM which was considered as the basal concentration for treatment of the cells during the whole steps of the work. Serving apoptotic morphological symptoms besides two dimensional plots of flow cytometric studies showed that percent value of apoptotic and necrotic cells in the medium have not had any significant difference with the negative control. On the other hand, no DNA laddering pattern observed for the drug-treated cells. These findings indicated that inhibitory effects of sodium sulfabenzamide on the viability of T-47D cell line should be exerted through some other non- apoptotic cellular processes. Here, prolongation of S and G2/M phases relative to the negative control (i.e. arresting of cell cycle in these phases) could partially explain the mechanism of action of sodium sulfabenzamide on T-47D cells.