مطالعه اثرات توكسيكوپاتولوژيك مصرف جلدي ديازينون بر پارامترهاي خوني و هيستوپاتولوژي در خرگوش سفيد نيوزلندي

Toxicopathologic effects of dermal exposure to Diazinon on hematological parameters and in rabbits organs

Diazinon [0, 0-diethyl 0-(2-isopropyl-6- methylpyrimidin-4yl) is an insecticide that belongs to a group of chemicals known as organophosphates. This research based on the toxicopathologic effects of dermal exposure of diazinon on male rabbits. Animals were divided randomly into two equal groups. Each group contains of six rabbits. The treatment group was given diazinon and the control group received ethyl alcohol, as solvent of the diazinon for 28 days. Blood samples were collected from both groups prior to the experiment (days 0,7th, 14th, 21st, and 28th) for hematological studies. The analysis of the obtained data suggests that diazinon has some effects on the hematological parameters of the experimental group. Significant decrease in the hemoglobin (Hb), hematocrit (Hct), and the red blood cell count observed in this group (p<0.05). The rabbits in both groups were euthanized after 28 days for histological studies. The tissues samples from heart, lungs, kidneys, stomach, and testis were fixed in 10% buffered formalin. 5 micron sections were stained in H&E. In treatment group histopathology revealed, hyperemia, emphysema, alveolar epithelialization and interstitial pneumonia in lungs. Other microscopic lesions which observed were tubular necrosis and hyaline cast in kidneys, atrophy and necrosis of muscle fibers in heart, degeneration in testis, and finally partial gastritis in stomach. The results show that diazinon has toxic effects on hematological and biochemical parameters and can able to produce pathologic changes in many organs of the body.