پديد آورندگان :
Tartibian، Bakhtiar نويسنده Department of Exercise Physiology, School of Physical Education, Department of exercise physiology, Urmia University, Urmia, Iran Tartibian, Bakhtiar , Baghaiee، Behrouz نويسنده Department of Exercise Physiology, School of Physical Education, Department of exercise physiology, Urmia University, Urmia, Iran Baghaiee, Behrouz , Baradaran، Behzad نويسنده Department of Immunology, Tabriz University of Medical Sciences, Immunology research center, Tabriz, Iran Baradaran, Behzad , Aliparasty، Mohammadreza نويسنده Department of Immunology, Tabriz University of Medical Sciences, Department of immunology, Tabriz, Iran Aliparasty, Mohammadreza , Almasy، Shohreh نويسنده Department of Immunology, Tabriz University of Medical Sciences, Department of immunology, Tabriz, Iran Almasy, Shohreh
كليدواژه :
oxidative stress , Super oxidase dismutase , Active people , Gene expression
چكيده لاتين :
Background: The purpose of this study is to compare Cu/Zn SOD mRNA and MDA level as a result of a session incremental exercise in active women and men.
Materials and Methods: This research is a quasi-experimental study with repeated measurements in which 14 active female and 13 male subjects with age range 22-24 participated voluntarily. Then, blood was taken from brachial vein of the subjects in three stages before and after GXT (Graded exercise test) and 3 hours after that and SYBER Green PCR Master mix reagent Kit and Real time-PCR were used to measure Cu/Zn SOD mRNA and spectrophotometer was used to measure MDA level.
Results: MDA levels increased significantly in men during the recovery stage and after the exercise (p1=0.012 and p2 =0.014), but it did not increase significantly in active women. Also, MDA difference between the two genders was not reported significant in any of the exercise stages. Cu/Zn SOD gene expression did not increase significantly in either sex.
Conclusion: The risk of injury from free radicals is more probable in active men than active women and vigorous physical activity does not significantly increase the Cu/Zn SOD gene expression.
