The Study of the Effects of Post Treatment with Rosmarinus Officinalis Aqueous Extract the First 48 Hours after Renal Ischemia/Reperfusion in Rats

Background: Acute renal failure induced by ischemia / reperfusion (I/R) causes excretory functional disorders of nephrons. The purpose of this study is to evaluate the intake of oral rosemary extract (gavage) on hemodynamic changes and tissue damages caused by I/R. Materials and Methods: This study was conducted on 40 Sprague-Dawley male rats, composed of 4 groups (n=10) including control, sham, I/R+normal saline (I/R+NS) post-treatment and I/R+rosemary (I/R+RO) post-treatment. They were fed with 10 ml/kg of 8% aqueous extract or vehicle as gavages during the first 48 hours after I/R. To induce I/R, both renal artery and vein were blocked for 30 minutes followed by a reperfusion within 48 hours. Then urine and plasma samples were collected and histological study was also conducted after removal of both kidneys. Data were analyzed by SPSS-11.5 software. The one-way analysis of variance (ANOVA) and Duncan test were used for intergroup comparison and p? 0.05 was used as the significant level. The Kruskal–Wallis multiple comparison test was also used for doing histological analysis Results: Comparison of renal function between two groups of (I/R+RO) and (I/R+NS), showed a significant reduction in plasma creatinine (p=0.05), the blood urea nitrogen (p=0.05), absolute excretion of sodium (p=0.001) and an increase in absolute potassium excretion (p=0.01). Results of the histological study also indicated a significant decrease in the vascular congestion, Bowman’s capsule space and oxidative stress in the group (I/R+RO) in comparison with (I/R+NS). Conclusion: Treatment with rosemary may be effective in the reduction of the functional and histological effects of I/R.