پديد آورندگان :
shayan، Sara نويسنده Department of Microbiology, School of Medicine, Zahedan University of Medical Sciences , , Bokaeian، Mohammad نويسنده , , Shahraki، Shahram نويسنده Department of Microbiology, Zahedan University of Medical Sciences, Zahedan, Iran Shahraki, Shahram , Saeidi، Saeide نويسنده Department of Biology, Faculty of Science, Islamic Azad University (Science and Resercher), Kerman ,
كليدواژه :
Allim sativum , Cefoxitin resistance , Escherichia coli , Lactamases , AmpC B
چكيده لاتين :
Background: AmpC beta-lactamases are capable to hydrolyse all beta-lactam antibiotics except cefepime and carbapenems. Herbal medicines have been important sources of products for the developing countries in treating common infectious diseases and overcome the problems of resistance and side effects of the currently available antimicrobial agents.
Materials and Methods: Total 410 non repetitive clinical E. coli strains recovered during 7 month, were screened for AmpC production by disc diffusion test using cefoxitin (30 µg) discs and confirmed by combined disc diffusion test using phenyl boronic acid and the MIC and MBC of Allim sativum (A. sativum) alcoholic extract against AmpC positive E. coli isolates were determined.
Results: A total of 107 of 410 isolates (26%) were cefoxitin resistant and 13 (3.1%) isolates harboured AmpC enzymes. A. sativum alcoholic extract were effective against AmpC producing E. coli isolates.
Conclusion: There is need for a correct and reliable phenotypic test to identify AmpC beta lactamases and to discriminate between AmpC and ESBL producers and also these bioactive plants may help alleviate the problem of drug resistance.
