بررسي جهش زايي و سرطان زايي نانوذرات بيسموت با استفاده از سالمونلا تيفي موريوم100TA و ميكروزوم كبد موش (9S)

Investigation about the Mutagenic and Carcinogenic Effects of Bismuth Nanoparticles with Using Salmonella typhimurium TA100 and Rat Liver Microsomes(S9)

زمينه و هدف: استفاده از نانو ذرات به علت ارزاني، مفيد بودن و خاصيت ضد ميكروبي بالاي آن ها، در زمينه هاي مختلف رو به افزايش است. بنابر اين بايد به مضرات احتمالي آن ها نيز توجه شود و در غلظتي استفاده شوند كه جهش زا و سرطان زا نباشد. لذا تحقيق حاضر با هدف بررسي اثرات جهش زايي و سرطان زايي نانوذرات بيسموت با سايز nm 56 و در سه غلظتppm 1/39، 1/78، 25/156 انجام گرفت. روش كار: در ابتدا جهش زايي ماده آزمون ايمز با استفاده از سالمونلا تيفي موريوم100TA سنجش و سرطان زايي آن جداگانه با ميكروزوم كبدي موش9S افزوده و شمارش كلني هاي برگشتي انجام گرديد. نتايج حاصل از اين آزمايش به كمك نرم افزار SPSS و روش آماري آناليز واريانس يك طرفه مورد بررسي قرار گرفت. يافته ها: در حضور ميكروزوم كبد موش تعداد كلني هاي برگشتي در مقايسه با عدم حضور اين آنزيم، كاهش يافته و اين بدين معني است كه اثر جهش زايي و سرطان زايي با حضور اين آنزيم كاهش مي يابد. نتيجه گيري: طبق نتايج پژوهش حاضر نانوذرات بيسموت فعاليت جهش زايي و سرطان زايي از خود نشان نداد.

Introduction & Objective: The usage of nanoparticles is growing in various fields due to their low cost, high efficiency and antimicrobial properties. Therefore, their potential hazards should also be considered and used at concentrations that have not mutagenic or carcinogenic effects. This study aimed to assess the mutagenic and carcinogenic effects of Bismuth nanoparticles with a size of 56nm, and the concentrations of 156.25,78.1, 39.1 ppm . Materials and Methods: In this study, we utilize the Ames test using Salmonella typhimurium TA100. Firstly, the purity of the strains was confirmed in terms of purity of mutagenic properties. Then the different concentration of Bismuth nanoparticles was separately added to minimal glucose agar medium containing TA100. Subsequently, they were compared against the positive control ( samples containing mutated bacteria and sodium azide ) and negative control ( containing mutated bacteria and distilled water ). In the next phase of this research the rat liver micrsomes was separately added to the minimal glucose agar medium containing the suspected carcinogenic Bismuth nanoparticles , and negative and positive controls and all back colonies were counted. Results: The number of revertant colonies the treated plates with S9 is decreased and it means mutagenic and carcinogenic effect of Bismuth nanoparticles with S9 is decreased. Conclusion: The results of the present study shows that the Bismuth nanoparticles at concentrations examined had no mutagenic and carcinogenic effect. Keywords: Bismuth Nanoparticles, Salmonella typhimurium 100, Ames Test, Microsomes