پديد آورندگان :
هاشم لو، اسماعيل نويسنده دانشجوي كارشناسي ارشد دانشكده كشاورزي، دانشگاه شهركرد، شهركرد , , جمالي زواره، عبدالحسين نويسنده استاديار دانشكده كشاورزي، دانشگاه شهركرد، شهركرد , , قوستا، يوبرت نويسنده استاديار دانشكده كشاورزي، دانشگاه اروميه، اروميه ,
چكيده لاتين :
In order to identify the endophytic fungi of stone fruit trees in West Azarbaijan province (Iran), healthy tissues were collected from various regions. For isolation of endophytic fungi, plant tissues were primarily washed with tap water, then surface sterilized by rinsing in 75% ethanol for 1 minute, followed by 5 minutes in 3% sodium hypochlorite (active ingredient). Disinfested pieces were then washed with distilled water, damp dried and plated onto culture media PDA and PCA (Schulz et al. 1993). Fungi growing from the margins of plant tissues were transferred separately into new culture media and purified by hyphal tip or single spore methods. After isolation of endophytic fungi, some isolates were identified as Sordaria fimicola (Roberge ex Desm.) Ces. & De Not. based (Guarro & von Arx 1987, Hanlin 1999) on morphological characters as follows: Colonies on MEA growing rapidly, 80 mm diam. in three days at 23–25o C, brown to grey, with radial furrows at margins. Hyphae hyaline to pale brown, aerial hyphae commonly formed. Perithecia formed after six days, scattered or aggregated, ostiolate, obpyriform, nonstromatic, glabrous, dark brown to black, more or less immersed in the substrate and surrounded by aerial mycelium, without stroma and setae, with long neck, 310–450 × 210–300 µm. Asci unitunicate, 8-spored, cylindrical, short stipitate, with truncate apex and thickened apical ring, hyaline, 162.5–192.5 µm long and 7–10 µm wide. Ascospores uniseriate, one-celled, ovoid to ellipsoidal, smooth with basal germination pore, yellow at first turning dark brown at maturity, with gelatinous sheath, (18–) 19–22 (–24) × (12–) 13–15 µm (Fig. 1).
Specimens examined: Iran, W Azarbaijan province, Miandoab-Mahabad road, Peach leaf, 30.9.2011,
E. Hashemloo, MMHS66I; W Azarbaijan province, Miandoab-Mahabad road, Peach shoot, 30.9.2011,
E. Hashemloo, MMHS65I (IRAN 2238 C);
W Azarbaijan province, Miandoab, Gavmish Goli village, Peach leaf vein, 30.9.2011, E. Hashemloo, MGHS73B.
