كليدواژه :
پزشكي , تري فلويوپرازين , Experimental diabetes , ديابت تجربي , Streptozotocin , نفوذپذيري عروق , Vascular disorder , Trifluoperazine
چكيده لاتين :
Chronic diabetes mellitus is associated with increased vascular permeability and closure of capillaries either in human or in experimental animal models. Since the events that lead to these vascular disorders have not been well-defined, therefore, the aim of the present study was to evaluate the beneficial effect of a calmodulin inhibitor, trifluoperazine (TFP) on the prevention of increased vascular permeability in an experimental model of chronic diabetic rat. This experimental study was carried out on female rats (200-250 g). The animals were randomly divided into 8 groups. Groups I to IV were normal and healthy animals, and groups V to Viii were diabetic. Groups II and VI received normal saline, groups Ill and VII received oral TFP, and TFP was injected to groups IV and VIII. Chronic diabetes was induced by subcutaneous injection of streptozotocin (50 mg/kg). Vascular permeability was determined by measuring the extra-vascular evans blue (E.B) dye and water contents of tissues 8 weeks after diabetes induction. The animals received TFP either orally (dissolved in drinking water) or by injection (8 mg/kg/day) subcutaneously during the study. The results of this study showed that E.B extravasation significantly increases by 61.2% in diabetic group (V) as compared to healthy group (I). Meanwhile, TFP injection inhibited the increased vascular permeability for E.B dye. On the other hand, E.B content was not significantly different in groups VIII and I, although the water content of tissues increased by 32% in group V as compared to group I. In addition, TFP either orally or by injection did not have any significant effect on the reduction of water content. Furthermore, oral TFP normalized hyperglycemia in diabetic animals. TFP also prevented weight loss in diabetic animals, whereas the weight loss that occurred in group V was not seen in groups VII and VIII. Water consumption of group VIII was not greater than group I. However, TFP, particularly its parenteral form increased E.B dye content, weight gain and water consumption in healthy animals. These results suggest that TFP as a calmodulin inhibitor exerts inhibitory effect on protein extravasation, weight loss, increased water consumption, and hyperglycemia in chronic diabetic rat.
