بررسي اثر محرك هاي شيميايي متيل جاسمونات و ساليسيليك اسيد بر تحريك توليد هايپرسين در گياه گل راعي (perforatum Hypericum)

Study the chemical stimuli effects of Methyl Jasmonate and Salicylic acid on stimulation of Hypericin production in Hypericum (Hypericum perforatum L.)

زمينه و هدف: در اين پژوهش، اثر محرك هاي شيميايي متيل جاسمونات و ساليسيليك اسيد در محيط كشت پايه (MS) بر تحريك توليد هايپرسين در يك دوره 42 روزه مورد بررسي قرار گرفت. مواد و روش ها: ريز نمونه هاي برگ و ساقه به محيط MS حاوي محرك هاي متيل جاسمونات و محرك ساليسيليك اسيد منتقل شدند. عصاره هاي متانولي اين گياه به وسيله دستگاه HPLC به منظور بررسي تغييرات در ميزان هايپرسين مورد تحليل قرار گرفتند. يافته ها : نتايج نشان دادند كه غلظت 100 ميكرومولار متيل جاسمونات نسبت به ساير غلظت ها بيش ترين ميزان هايپرسين (66/34%) را توليد مي كند. هم چنين نيز استفاده از غلظت هاي بيشتر از 100 ميكرومولار ساليسيليك اسيد باعث از بين رفتن نمونه هاي كشت بافتي گياه گل راعي مي شود. نتيجه گيري: محرك شيميايي متيل جاسمونات در افزايش ميزان هايپرسين در كشت بافت گل راعي بسيار تاثير داشته است. اما محرك شيميايي ساليسيليك اسيد تاثير محسوسي در افزايش ميزان هايپرسين در گياه گل راعي نداشت

Aim and Background: Hypericin is a photosensitive Naphthodianthrones with photodynamic activities. In recent years, the pharmacology studies have shown that extracts and compounds obtained from Hypericum perforatum have some properties such as: antidepressants, anti-cancer, anti-antioxidant, anti-bacterial and anti-microbial and recently, there is great attention to this herb due to its effect for treatment of AIDS. In this work, the chemical stimuli effects of methyl-jasmonate and salicylic acid in medium stage (MS) on Hypericin production stimulation during a 42-day period was examined. Material and Methods: Stem and leaf explants were isolated from planted sterile seedlings in medium and were transferred to MS condition including methyl-jasmonate stimulus with concentration of (0, 50, 100, 150, 250 and 500 µicro-Molar) and salicylic acid stimuli with concentration of (0, 50, 100, 150, 200 and 400 µicro-Molar) and then were kept in temperature about 21 ± 3 °C in 16 hours lighting and 8 hours dark light condition. The methanol extracts of this plant were analyzed with HLPC apparatus for studying the changes in Hypericin amount. Conclusion: The results showed that the concentration of 100µM methyl- jasmonate produces more Hypericin (%34, 66) than other producers. Also these results showed that salicylic acid stimuli have no tangible effect on Hypericin production and using concentrations more than 100 µM of salicylic acid causes destruction of plant tissue cultures of Hypericum perforatum. Results: The chemical stimulus, methyl -jasmonate is more effective in Hypericum production of plant Hypericin tissue culture. The chemical irritant effect of salicylic acid did not significantly increase the amount Hypericin in the plant Hypericum.