مقايسه روش هاي جداسازي بيوفيلم هاي باكتريايي تشكيل شده بر سطوح لوله هاي انتقال آب و وسايل استفاده شده در شيردوشي در دامداراي ها

Comparing Methods of Separating Bacterial Biofilms on the Surface of Water Transportation Pipes and Equipment of Milking in the Farms

بیوفیلم های باكتریایی بسته به تركیب و محل ایجاد می توانند سودمند یا زیان آور باشند. تشكیل آنها در لوله های انتقال آب و استفاده شده در شیردوشی در دامداری ها می تواند مشكلاتی را ایجاد نمایدو منبع بالقوه ای برای آلودگی باشد. در این تحقیق با توجه به توانایی تشكیل بیوفیلم توسط باكتری با سیلوس سوبتیلیس با برخورداری از خصوصیات ویژه انتخاب گردید. ابتدا امكان تشكیل بیوفیلم بر روی قطعات مختلف پلاستیك (پلی وینیل كلراید، پلی پروپیلن و پلی اتلین گیكول)، فلزآلومینیوم و شیشه در دمای 4،30،37 درجه سانتیگراد مورد مطالعه قرار گرفت. بر روی تمام سطوح مورد استفاده بیوفیلم با سیلوس سوبتیلیس تشكیل شد. و دمای بهینه تشكیل بیوفیلم آن 30 درجه سانتیگراد تعیین گردید. دو روش جداسازی بیوفیلم ها شامل زدودن با سوآپ و ورتكس بكار رفت و بازده هر روش محاسبه گردید. روش ورتكس بازده و كارایی بیشتری برای جداسازی سلولهای چسبیده و بیوفیلم-ها از سطوح مختلف داشت. شمارش تام پرگنه های حاصل از بیوفیلم مشخص كرد كه احتمال چسبیدن باكتریها به سطوح پلاستیكی به خصوص پلی پروپیلن بیشترین و سطوح شیشه ای كمترین بود.در واقع چسبیدن سلول های باكتری با توجه به خاصیت فیزیكی سطوح به نسبت 2-2/1 برابر ممكن است متغیر باشد .نتایج این مطالعه می تواند به انتخاب لوله های مناسب برای انتقال مایعات بویژه آب و كاهش آلودگی در دامداری ها كمك كند.

Introduction Bacterial biofilms can be both useful and harmful based on their combination and locations. Biofilm formation occurs as a stepwise process. Their formation in liquid transportation pipes used for milking system and drinking water in animal farms may create some problems and is a potential source of pollution. Speed of biofilm formation depends on many factors including: construction and functional characteristics of bacteria, the composition and culture conditions such as temperature and substratum. In this research the Bacillus subtillis bacteria with special characteristics was selected due to its capability for biofilm creation. Bacillus subtillis bacteria is mobility and a stronger connection than other bacteria levels are created. In the research conducted in the biofilm there are many resources on biofilm formation by Bacillus subtillis bacteria. Bacillus subtillis is saprophytic in the soil, water and air. There is also the ability to form spores of Bacillus subtillis. Materials and Methods Firstly the possibility of creating biofilms on different Plastic (polyvinilchlorid, polypropylene, polyethylengelycole), alluminum and glass surfaces in three temperatures of 4°C, 30°C and 37°C were studied. Two different methods of biofilms separation including separating swap and vortex were tested and their efficienceies were calculated. After biofilm formation on parts of the vortex separation method after washing parts in sterile conditions in a tube containing normal saline for 4 minutes was vortex. The bacterial suspension decreasing dilution series was created. Pour plate in medium using agar plate count agar and was cultured at 30°C for 24-48 hours. Numbers of colonies were counted. The numbers of biofilm cells were calculated. In swap method after biofilm formation on parts using a cotton swap was isolated biofilms. The swap was transferred to tube containing normal saline and the bacterial suspension decreasing dilution series was created. Pour plate in medium using agar plate count agar and was cultured at 30°C for 24-48 hours. Numbers of colonies were counted. The numbers of biofilm cells were calculated. Results and Discussion Bacillus subtillis biofilms were formed on all studied surfaces. Total count of bacteria detached from biofilm indicates that these bacteria can develop on the polypropylene surface much more than the other surfaces. 10 days later, the formation of biofilm reached the maximum level. The optimum temperature was 30°C. The vortex method was more efficient in comparison to other methods. The bacterial attachment was highest with the plastic surface, specially the propylene surface; whereas the lowest attachment was detected in the glass surfaces. Synthetic materials based on hydrocarbons are more susceptible to the formation of biofilm and infection. Generally lower levels are microscopic pores and also less likely to form a biofilm. Rusty pipes, old, worn-out and scratched plastic furniture raised the possibility of contamination and could cause problems. Conclusion It was concluded that glass pipes are the best materials for liquid transportation in different forms of animal farms. According to this study, basic methods of removing biofilm and enable ranchers to make good use of the equipment and the practical methods of removing contaminants help.