ارزيابي تغييرات جريان خون مغزي در موش صحرايي وابسته به مورفين در طول نشانگان ترك ناشي از نالوكسان

Cerebral Blood Flow Measurements During Naloxone-Precipitated Morphine Withdrawal in Dependent Rats

Laser-Doppler flowmetry (LDF) was used to measure changes in regional cerebral blood flow (rCBF) following morphine and naloxone administration in morphine-dependent anesthetized rats. Morphine (10 mg/kg; i.p.) administration reduced rCBF in control, sham-operated and morphine-dependent rats, but the depressant effect of morphine in morphine-dependent animals was less than that of controls and sham-operated groups. While naloxone (0.5 mg/kg; s.c.) had no considerable effect on rCBF in control and sham-operated groups, it increased rCBF in morphine dependent ones. The depressant effect of morphine in the naive group and the enhancing effect of naloxone in morphine-dependent animals were not seen after local application of lidocaine upon the recording site. This study may provide a framework to study the cellular and molecular mechanisms responsible for coupling neuronal electrical activity with regional alteration in blood flow during precipitation of morphine withdrawal.