اثر پيش تغذيه روغن دانه كتان (Linum usitatissimum) بر نفوذپذيري سدخوني مغزي در مدل سكته مغزي موش صحرايي

The effect of prefeeding flaxseed oil (Linum usitatissimum) on blood brain barrier permeability in rat stroke model

ادم ايسكميك مغزي از مهمترين عوارض سكته مغزي مي‎باشد. هدف از اين مطالعه بررسي اثر پيش تغذيه روغن دانه كتان بر نفوذپذيري سد خوني مغزي در مدل سكته مغزي موش صحرايي مي‎باشد. در اين تحقيق تجربي حيوانات به طور تصادفي به 3 گروه آزمايشي، شم و كنترل تقسيم گرديدند كه گروه آزمايشي خود شامل 3 گروه دريافت‎كننده روغن دانه كتان با دوزهاي 25/0، 5/0 و 75/0 ميلي‎ليتر بر كيلوگرم وزن بدن به مدت 30 روز بود. گروه كنترل آب مقطر دريافت مي‎كردند، در حالي كه براي گروه شم تيمار و القاي ايسكمي صورت نميگرفت. دو ساعت بعد از آخرين دوز خوراكي گروهها تحت جراحي مدل MCAO (انسداد شريان مياني مغز) به منظور القاي ايسكمي و بررسي ميزان نفوذپذيري سد خوني مغزي از طريق اندازه‎گيري ميزان جذب نوري اوانس آبي (Evans Blue) قرار گرفتند. داده‎ها با استفاده از آزمون ANOVA تجزيه و تحليل شدند. كاهش نفوذپذيري سد خوني مغزي در گروه هاي با دوز 5/0 و 75/0، در برابر گروه شاهد از لحاظ آماري معني‎دار بود (05/0>P).

Abstract Ischemic Brain Edema is one of the most important complications of a stroke. This study aims to investigate the impact of prefeeding of flax seed oil on bloodbrain barrier in rat stroke model. In this experimental study animals were randomly divided into 3 groups of experiment, sham, and control. Each testing group itself was consisted of 3 subgroups receiving doses of Flaxseed oil with 0.25, 0.5 and 0.75 ml / kg of body weight for 30 days. The control group received distilled water, while therapy and ischemia induction were not done for sham group. In order to conduct ischemia induction and evaluating the permeability of the bloodbrain barrier by measuring the optical density level of the Evans Blue MCAO (Middle Cerebral Artery Occlusion) model surgery was conducted on groups two hours after the last feeding dose. The data were analyzed via ANOVA test. The permeability decrease of the bloodbrain barrier in groups with doses of 0.5 and 0.75 ml against the control group was statistically significant (P˂0/05). There were significant differences between the right and left hemispheres in control groups with dose of 0.25ml (P˂0/05).But the differences between the right and left hemispheres in therapy group with doses of 0.5 and 0.75 were insignificant. According to the findings of this study, pretreatment with flax seed oil can reduce the permeability of the bloodbrain barrier in a model of ischemiareperfusion in rats. But still more works to extend these observations including the investigation of neurological deficits are needed to be done.