بررسي اثر كافيين بر روي سلولهاي ماكروفاژ ريوي موش صحرايي القاء آپوپتوزيس توسط كافيين

Effects of Caffeine on Alveolar Macrophages From Rat Lungs:Caffeine Induced Apoptosis

In this study, alveolar macrophages were lavaged from rat lung, exposed to two concentrations of caffeine and incubated for 24 hours at standard condition. The effect of caffeine was assessed by measuring percent of viable cells, DNA fragmentation and superoxide anion production. The results show that the effects of caffeine was dose dependent. The viability of the cells, at low concentrations of caffeine (<5 mM) increased remarkably with a peak at 2.5 mM. Analysis of anion superoxide production and DNA fragmentation showed no significant increase. At moderate concentrations of caffeine (5-20mM), the viabilty was higher than the control, while at higher concentrations (>20 mM) significant reduction in the cell survival and a significant rise in both anion superoxide production and DNA fragmentation were observed. These results suggest that low concentrations of caffeine prevents apoptosis of alveolar macrophages where at moderate concentrations induces apoptosis and at high concentrations necrosis has occurred.